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glucose free dmem medium (Thermo Fisher)

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Thermo Fisher glucose free dmem medium
Glucose Free Dmem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+free+dmem+medium/D(%2B)-Glucose/pmc12913883-307-15-18
Average 99 stars, based on 1 article reviews

glucose free dmem medium - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Modelling diabetes-associated metabolic stress in human multicellular cardiac microtissues.
Article Snippet: Type 2 diabetes is a global health crisis, closely associated with an increased risk of heart failure due to the development of diabetic cardiomyopathy.. Progress in understanding the underlying mechanisms and identifying effective treatments has been limited by the lack of robust preclinical models that accurately mimic human cardiac physiology.. Human induced pluripotent stem cells (iPSCs) offer the unique ability to generate large quantities of both cardiomyocytes and non-myocytes, enabling the development of advanced models for cardiovascular research.

Article Title: Long-read sequencing-based atlas of tissue-specific expression of Drp1 transcript variants
Article Snippet: From days 14 to 19, cardiomyocytes were enriched to >90% cardiac troponin T positive cells by culture in glucose-free DMEM medium (Thermo Fisher Scientific) containing 4 mM sodium L-lactate (Sigma-Aldrich).

Article Title: Epigenetic and metabolic rewiring in metastatic pheochromocytomas and paragangliomas driven by SDHB mutations
Article Snippet: After 24 h, once the cells adhered, the medium was removed, and 100 μl of glucose-free DMEM medium (Gibco, ThermoFisher) without supplements was added to each well.

Article Title: Epigenetic and metabolic rewiring in metastatic pheochromocytomas and paragangliomas driven by SDHB mutations.
Article Snippet: After 24 hours, once the cells adhered, the medium was removed, and 100 μl of glucose-free DMEM medium (Gibco, ThermoFisher) without supplements was added to each well.

Incubation:

Article Title: Long non-coding RNA THAP7-AS1 interacts with EIF3A and enhances ITPR1 to enhance endoplasmic reticulum stress and endothelial cell pyroptosis in ischemic stroke.
Article Snippet: .. Specifically, HUVECs in the logarithmic growth phase were replaced with glucose-free DMEM medium (Gibco) and incubated in an anaerobic chamber flushed with a 95% N2 and 5% CO2 gas mixture for 6 h to simulate an ischemic-hypoxic microenvironment. ..

Positive Control:

Article Title: Evaluation of Potential Anti-Diabetic Synbiotic Formulation of Lacticaseibacillus rhamnosus BST.L-601 Using db/db Mice
Article Snippet: .. The cells were treated with heat-killed BST.L-601 (5 × 10 7 to 5 × 10 8 CFU/mL) in glucose-free DMEM medium (Gibco, Grand Island, MA, USA) for 16 h. As a positive control group, insulin (100 nM) was treated under the same conditions. ..

Cell Culture:

Article Title: GOT1 Inhibition Induces Extracellular Matrix Remodeling in Pancreatic Cancer.
Article Snippet: Cell-containing hydrogel solutions were dispensed as droplets of 37.5 μL on a Sigmacote (Sigma SL2)-treated glass slide and crosslinked at 405 nm for 4 min. Hydrogel constructs were transferred to non-treated 48- well plates containing 500 μL of PDAC or TKKC cell medium. .. For glucose studies, cell-laden hydrogels were cultured using 500 μL of glucose-free DMEM medium (Gibco 11966025). ..

Concentration Assay:

Article Title: Mitochondrial crosstalk between bone marrow stromal cells and chondrocytes: implication for cartilage repair in osteoarthritis.
Article Snippet: Purpose/Aim: Mitochondria are vital dynamic organelles released by cells into extracellular space, endocytosed in or transferred between cells in contact.. Mitochondria from healthy bone marrow stem cells (MSCs) show rescue effects on chondrocytes, accordingly a concept of using healthy MSC mitochondria for cartilage regeneration is put forward.. Therefore, whether mitochondria from healthy MSCs help to save chondrocytes in damaged cartilage microenvironment is intriguing.

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Mutant ND4 led to an imbalance of oxidative stress. The 661 W cells cultured in galactose or high glucose DMEM were stably expressing exogenous ND4. The levels of ROS ( A , B ) were measured. Under galactose conditions, an increase in ROS was evident in 661 W cells. Furthermore, cells expressing exogenous m.11778G > A genes displayed exacerbated changes. ( P = 0.0212, P< 0.0001, n = 6 per group). Scale bar = 100 μm. Data is presented as means ± SEM, n = 4, one-way ANOVA. **** P < 0.001.

Journal: Scientific Reports

Article Title: Oxidative stress imbalance and cellular damage mediated by the ND4 G11778A mutation

doi: 10.1038/s41598-026-40061-0

Figure Lengend Snippet: Mutant ND4 led to an imbalance of oxidative stress. The 661 W cells cultured in galactose or high glucose DMEM were stably expressing exogenous ND4. The levels of ROS ( A , B ) were measured. Under galactose conditions, an increase in ROS was evident in 661 W cells. Furthermore, cells expressing exogenous m.11778G > A genes displayed exacerbated changes. ( P = 0.0212, P< 0.0001, n = 6 per group). Scale bar = 100 μm. Data is presented as means ± SEM, n = 4, one-way ANOVA. **** P < 0.001.

Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM, high glucose, BIOEXPLORER, Cat#B1101-001); DMEM glucose-free medium (Basalmedia, Cat#L160KJ); Fetal bovine serum (FBS, Capricorn, Cat#F12A); Penicillin and streptomycin (Gibco, Cat#15140122); Galactose (Sigma-Aldrich, Cat#G5388); Puromycin (Basalmedia, Cat#S250J0); Cell-counting kit 8 (CCK-8) (Biosharp, Cat#BS350A); ROS assay kit (Solarbio, Beijing, China, Cat#CA1410); Superoxide Dismutase Assay Kit with WST-8 (Beyotime, Cat#S0101S); Catalase (CAT); Assay Kit (Solarbio, Cat#BC0205); GSH and GSSG Assay Kit (Beyotime, Cat#S0053); One-step TUNEL Apoptosis Kit (Elabscience, Cat#E-CK-A322); DAPI (Beyotime, Cat#C1005); Hoechst 33,342 (Solarbio, Cat#C0030); Seahorse XFe24 microplates (Agilent, Cat#02340); Agilent Seahorse XF DMEM medium (Agilent, Cat#103015); Sodium pyruvate (Agilent, Cat#103578); Glutamine (Agilent, Cat#103579); D-glucose (Agilent, Cat#103577); BCA protein assay kit (Beyotime, Cat#P0010); Mitochondrial Extraction Kit ( solarbio, Cat: SM0020); Blue/Clear Native-PAGE Gel Quick Preparation Kit (Meilunbio, MA0470); Acetylcysteine ( MedChemExpress, Cat#HY-B0215 ).

Techniques: Mutagenesis, Cell Culture, Stable Transfection, Expressing

Mutant ND4 led to a decrease of antioxidative stress. The 661 W cells cultured in galactose or high glucose DMEM were stably expressing exogenous ND4. The activity of the enzymes SOD ( P = 0.0079, P <0.0001, n = 6 per group) ( A ) and T-GSH ( P = 0.0044, n = 4 per group) ( B ), and the level of CAT ( P = 0.0442, n = 4 per group) ( C ) were measured. Under high-glucose conditions, the activity of SOD and T-GSH were significantly reduced in 661 W cells. Under galactose conditions, the activity of SOD was significantly reduced in 661 W cells. Furthermore, cells expressing exogenous m.11778G > A genes displayed exacerbated changes. Scale bar = 100 μm. Data is presented as box and whiskers, one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Scientific Reports

Article Title: Oxidative stress imbalance and cellular damage mediated by the ND4 G11778A mutation

doi: 10.1038/s41598-026-40061-0

Figure Lengend Snippet: Mutant ND4 led to a decrease of antioxidative stress. The 661 W cells cultured in galactose or high glucose DMEM were stably expressing exogenous ND4. The activity of the enzymes SOD ( P = 0.0079, P <0.0001, n = 6 per group) ( A ) and T-GSH ( P = 0.0044, n = 4 per group) ( B ), and the level of CAT ( P = 0.0442, n = 4 per group) ( C ) were measured. Under high-glucose conditions, the activity of SOD and T-GSH were significantly reduced in 661 W cells. Under galactose conditions, the activity of SOD was significantly reduced in 661 W cells. Furthermore, cells expressing exogenous m.11778G > A genes displayed exacerbated changes. Scale bar = 100 μm. Data is presented as box and whiskers, one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques: Mutagenesis, Cell Culture, Stable Transfection, Expressing, Activity Assay

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